” A “shaker” is the device used to perform vigorous shaking

There are various types of shakers

It is used to shake vessels such as test tubes and flasks horizontally or vertically to mix or react substances within samples. It is also used for culturing microorganisms as well as plant and animal cells. It is utilized across various fields and applications.

Applications and Fields of Use for Shakers

The primary fields and applications of shakers include the following:

Various Shaking Methods

There are various shaking methods depending on the purpose and application.
The primary methods are as follows:

Shaking methods	Description	Illustration	Lineup (Shaking method)
Reciprocal shaking (Horizontal shaking)	This is the most common shaking method, utilizing a side-to-side reciprocating motion. It is suitable for a wide range of applications such as reactions, dissolutions, and culturing. Thanks to its excellent aeration performance, it is also ideal for pre-culturing yeast and for culturing stressed E. coli cells after transformation.		MMS-1020（Reciprocal） MMS-120H（Reciprocal+High-humidity） MMS-3021（Reciprocal※） ※For testing of food allergens and residual pesticides MMS-3020（Reciprocal/Orbital） MMS-320（Reciprocal/Orbital） MMS-4020（Reciprocal/Orbital/Figure-8） MMI-1000A・B（Reciprocal/Orbital） MRM-1000（Vertical rotation/reciprocal /Reciprocal+vibration） NTS-4000（Reciprocal）
Orbital shaking (Rotary shaking)	This shaking method follows a circular motion. Compared to reciprocating shaking, it is said to impose less physical stress on samples, making it suitable for a wide range of applications such as reactions, dissolutions, and culturing. It is particularly used in culturing experiments for plants and microorganisms, as well as for DNA extraction and protein production.		MMS-220H（Orbital＋High-humidity） MMS-3020（Reciprocal/Orbital） MMS-320（Reciprocal/Orbital） MMS-4020（Reciprocal/Orbital/Figure-8） MMS-520（Orbital+Powerful shaking） MMS-5020（Orbital+Powerful shaking） MRM-1000（Vertical rotation/reciprocal/Reciprocal＋vibration） CM-1000（Orbital+High-speed）
Figure-8 shaking	This model utilizes a combination of reciprocating and orbital shaking motions, offering superior mixing and agitation efficiency. It enables uniform and effective shaking, making it ideal for dissolution, stirring, and mixing in chemistry-related fields. It is also well-suited for molecular biology applications such as gel and membrane washing, ELISA reactions, and Western blotting procedures.		MMS-420（Figure-8） MMS-4020（Reciprocal/Orbital/Figure-8）
Vertical shaking	This type of shaker moves the samples vertically up and down. It offers the highest efficiency for mixing and agitation, making it ideal for liquid-liquid extraction and the blending of powders. It is widely used in sample pretreatment processes such as extracting food, soil, and environmental contaminants using separatory funnels.		MMV-1000W（Vertical/Inclined）
Single-axis shaking	This shaking method involves vertical arc motion and is suitable for L-shaped test tubes and vials. The high contact efficiency between liquid and air makes it ideal for microbial cultivation and BOD (Biochemical Oxygen Demand) testing.		—
Seesaw-type shaking	This shaking method gently rocks the platform up and down like a seesaw, providing uniform and mild agitation. It is commonly used in temperature-controlled equipment such as hybridization ovens for DNA/RNA hybridization. Typical applications include placing hybridization bags or plastic containers for gel staining and destaining on the platform.		—

Video Explaining Different Shaking Methods

Temperature Control Range

The temperature control range of each shaker is as follows.

How to Select Optional Shaking Platforms ＆ holders (Multi-Purpose Shaker MMS Series)

Optional shaking platforms and holders should be selected based on the vessels used, the number of samples, and the specific application.

For shaking with various vessels:
→ Universal Shaking Platform with Spring (Models MMS TRAY-S/L)

For shaking fixed-volume flasks:
→ Multi-Shaking Platform (Models MMS BASE-S/L)

Various Flask Holders:

For shaking Petri dishes:
→ Adhesive Mat Platform (Models MMS FIT-S/L)

For shaking centrifuge tubes:
→ Centrifuge Tube Platform (Models MMS BASE-15/50)

What is a Incubator Shaker?

Incubator shakers are commonly used in research and culture settings. While the name is familiar, there may be few opportunities to reconsider the specific role this equipment plays.
A incubator shaker is a device capable of shaking vessels while maintaining a constant temperature. Its key feature is the ability to perform “temperature management” and “mixing/culture” simultaneously.

For example, in “microbial and cell culture”:
Experiments involving the culture of organisms such as E. coli, yeast, animal cells, and plant cells require a constant temperature while ensuring a uniform distribution of air and nutrients. A incubator shaker, which can stably create such an environment, is an indispensable asset for culture experiments.

EYELA offers both types of shakers; one with a separate constant temperature chamber and an integrated constant temperature shaker. There are advantages to having a fully integrated system, as well as distinct benefits to the traditional separate configuration. Below are the detailed merits of both the integrated and separate models.

Product	Incubator shaker (Integrated)	Shaker + Constant Temperature Chamber (Separated)
		Shaker	Constant Temperature Chamber
Model	MMI-1000A	MMS-320	FMS-100
Cat. No.	281790	267830	191060
Shaking method	Reciprocal/Orbital	Reciprocal/Orbital	—
Shaking speed	20 to 250rpm	20 to 250rpm	—
Amplitude	25mm	25mm	—
Temperature control range	RT + 5 to 60℃	—	RT + 5 to 50℃
Temperature control accuracy	Within ±0.5°C	—	±0.2°C

Merits of the Integrated Incubator Shaker MMI-1000A

When using the universal shaking platform with spring, MMA-1000A can accommodate up to two 1L Erlenmeyer flasks. The reciprocal and orbital shaking modes can be easily toggled with a single lever.

• Lower cost compared to combining a shaker with a constant temperature chamber
• Temperature control up to +60°C
• Space-saving, compact size suitable for use on laboratory benches
• Large opening for smooth sample loading and unloading
• Transparent hood allows for easy monitoring of shaking status at a glance

Merits of the Separated Shaker MMS-320 + Constant Temperature Chamber FMS-100

MMS-320 shaker supports both reciprocal and orbital shaking. Shaking modes can be easily switched with a single-touch operation using the front lever. FMS-100 constant temperature chamber can accommodate shakers with dimensions up to 400W × 340D mm. When installed, the shaker can be used with Erlenmeyer flasks up to 2L in size.

• Effective internal height of 470mm; allows use of Erlenmeyer flasks up to 2L on the installed shaker.
• Can be used as a standalone incubator without installing a shaker.
• Low overall height even with a shaker inside, allowing for installation under laboratory benches.
• The internal bottom shelf slides forward and backward, and the full-sized front hood cover is a flip-up type, ensuring smooth loading and unloading of shakers and culture vessels.

Ideal Mixer for High-Speed Shaking of Micro-Samples such as Microtubes

High-Speed Plate Mixer, Model MMP-1000

*Optional shaking racks are sold separately. Vessels are not included.

MMP-1000 handles high-speed mixing for a wide variety of vessels

By simply changing the shaking racks, the unit supports shaking for microtubes, microplates, and deep-well plates. The shaking racks feature a one-touch attachment/detachment mechanism for easy replacement.

Product
Model	MP-R0.5	MP-R1.5	CM-RP	CM-RD
Cat. No.	286230	286240	188190	192310
Rack type	Microtube rack 0.5mL	Microtube rack 1.5/2.0mL	Microplate rack (stackable up to 2 units)	Deepwell microplate rack
Max. number of vessels	24 pcs/rack	24 pcs/rack	2 plates/rack	1 plate/rack
Max. number of racks	4 pcs	4 pcs	8 pcs	4 pcs
Applications	・Preparation of buffers, reagents, and enzyme solutions ・Cell suspension and lysis buffer mixing ・Enzymatic reactions for DNA/RNA extraction, etc.		・Acceleration of antigen-antibody reactions (e.g., ELISA) ・Biopanning of phage display libraries ・Low volume high-throughput culture of E. coli and yeast, etc.

Can be used inside an incubator

With an operating ambient temperature range of 4 to 50°C, this unit can be used inside FMC/FMS series shaker chambers or LTE series incubators. It is suitable for applications such as accelerating enzymatic reactions in plates or performing shaking cultures.

By using the optional communication cable and dedicated software, you can control the start/stop functions, shaking speed, and timer settings from a PC while the unit is installed inside the chamber.
*The dedicated software can be downloaded from our website.